Messi, Mascherano and Higuain missing for Argentina
Messi, 26, was ruled out for three weeks after pulling his thigh in the La Liga win over Almeria.
Defensive player Mascherano, 29, meanwhile injured his hamstring when Barca beat Real Sociedad.
These absences from Alejandro Sabella’s roster are offset by the fact Argentina have already qualified for the finals next summer.
Former Liverpool winger Maxi Rodriguez is one of six domestic-based players named.
Argentina squad for Peru and Uruguay matches: Sergio Romero, Mariano Andujar, Agustin Orion, Fabricio Coloccini, Pablo Zabaleta, Federico Fernandez, Marcos Rojo, Ezequiel Garay, Hugo Campagnaro, Sebastian Dominguez, Jose Maria Basanta, Cristian Ansaldi, Gabriel Mercado, Maxi Rodriguez, Angel Di Maria, Ever Banega, Lucas Biglia, Rodrigo Brana, Augusto Fernandez, Ricky Alvarez, Erik Lamela, Leandro Somoza, Sergio Aguero, Ezequiel Lavezzi, Rodrigo Palacio, Maurco Icardi.